Research Highlights

In the face of climate change, breeding strategies that can improve crop resilience and productivity across a range of environments is increasingly important, but these approaches require sufficient knowledge of the genes that underlie productivity and adaptation. By studying 732 genotypes grown at 10 experimental gardens in eight states spread across 1,100 miles, a large team of researchers have produced a high-quality reference sequence of the complex switchgrass genome.

GLBRC scientists have established a machine learning model to help explain variation in nitrous oxide emissions from agricultural soils. Trained on data collected from GLBRC field sites in Michigan and Wisconsin, the tool can predict nitrous oxide fluxes much more accurately than existing models.

The most promising microbes for making fuels and chemicals from plant material have broad appetites and biosynthetic potential but have not been widely studied in research labs. In three recent studies, scientists report crucial information on how to control genes in unique and promising biosynthetic microbes.

Recently converted farmlands aren’t as productive as traditional food cropping systems, but cultivating bioenergy feedstocks could provide alternative revenue sources for farmers, reduced competition with food production, and better maintenance of water quality due to soil fixation by root systems.

GLBRC researchers engineered Z. mobilis strains for the ability to ramp down levels of a key ethanol-producing enzyme. This functions as a switch, allowing carbon to be funneled into other bioproducts at high efficiency.

GLBRC researchers used small organic thiols (sulfur-containing compounds) to mimic enzymes from wood-digesting bacteria in an attempt to cleave the β-O-4 bond, the most common linkage in lignin. This thiol-based strategy could be a viable way to break down lignin and enable further processing to turn it into valuable chemicals and other bioproducts.

GLBRC researchers modeled soil carbon changes in switchgrass plantings in Midwestern experimental sites with varied soil composition under three climate change scenarios. Their findings suggest that switchgrass-derived biofuels can lead to a net decrease in carbon emissions. However, the size of this carbon benefit will be affected by climate parameters as well as plant biomass production and soil characteristics.

GLBRC researchers identified the genes responsible for xyloglucan synthesis in Arabidopsis and showed that, surprisingly, xyloglucan is not necessary for plant growth and development. These results raise important questions regarding cell wall structure and its reorganization during growth.

GLBRC scientists measured the growth and nitrogen cycling of 12 cultivated varieties of switchgrass to understand the strategies that different cultivars use to acquire and conserve nitrogen. Results suggest substantial nitrogen cycle differences in switchgrass that could be harnessed to create new or improved high-yielding, nitrogen-conserving cultivars.

GLBRC researchers have deciphered the biosynthesis of particular furan fatty acids in two species of α-proteobacteria by identifying the genes necessary and sufficient for the production of these furan fatty acids. Gas chromatography–mass spectrometry and nuclear magnetic resonance spectroscopy were used to identify the chemical structures of the products and intermediates of this pathway, and isotopic studies were conducted to determine the source of the oxygen atom in these furan fatty acids.