Complete genome sequence and the expression pattern of plasmids of the model ethanologen Zymomonas mobilis ZM4 and its xylose-utilizing derivatives 8b and 2032
S. Yang et al. (2018) Complete genome sequence and the expression pattern of plasmids of the model ethanologen Zymomonas mobilis ZM4 and its xylose-utilizing derivatives 8b and 2032. Biotechnology for Biofuels 11: 125
Zymomonas mobilis is a natural ethanologen being developed and deployed as an industrial biofuel producer. To date, eight Z. mobilis strains have been completely sequenced and found to contain 2–8 native plasmids. However, systematic verification of predicted Z. mobilis plasmid genes and their contribution to cell fitness has not been hitherto addressed. Moreover, the precise number and identities of plasmids in Z. mobilis model strain ZM4 have been unclear. The lack of functional information about plasmid genes in ZM4 impedes ongoing studies for this model biofuel-producing strain. In this study, we determined the complete chromosome and plasmid sequences of ZM4 and its engineered xylose-utilizing derivatives 2032 and 8b. Compared to previously published and revised ZM4 chromosome sequences, the ZM4 chromosome sequence reported here contains 65 nucleotide sequence variations as well as a 2400-bp insertion. Four plasmids were identified in all three strains, with 150 plasmid genes predicted in strain ZM4 and 2032, and 153 plasmid genes predicted in strain 8b due to the insertion of heterologous DNA for expanded substrate utilization.
The complete genomic sequences have been deposited in GenBank under accession number CP023715 for ZM4 chromosome and its four plasmids: CP023716 for pZM32, CP023717 for pZM33, CP023718 for pZM36 and CP023719 for pZM39; CP023677 for 2032 chromosome and its four plasmids: CP023678 for pZM32, CP023679 for pZM33, CP023680 for pZM36 and CP023681 for pZM39; and CP023682 for 8b chromosome and its four plasmids: CP023683 for pZM32, CP023684 for pZM33, CP023685 for pZM39 and CP023686 for pZM41.