Enhanced Biomass Digestion with Wood Wasp Bacteria

Cameron Currie, Brian Fox, Taichi Takasuka, Adam Book

Plant biomass represents a vast and renewable source of energy. However, harnessing this energy requires breaking down tough lignin and cellulose cell walls. In nature, certain microbes can deconstruct biomass into simple sugars by secreting combinations of enzymes. Two organisms that utilize cellulose are Clostridium thermocellum and Trichoderma reesei. Both are well-known and relied upon in the biomass field. Yet research suggests another microorganism, of the Streptomycesbacteria group, may hold previously unrecognized potential. Streptomyces species ActE is associated with a destructive wood-eating wasp and could represent a new source of cellulose-degrading enzymes.

The Invention

UW–Madison researchers have derived preparations from ActE secretions that highly degrade lignocellulose. The bacteria can be obtained from Sirex noctilio wasps and grown on a substrate containing mostly cellulose, hemicelluloses, xylan, wood or non-wood biomass, and chitin. The substrate may be pretreated for better results. The ActE are grown aerobically to maximize the secretion of both oxidative and hydrolytic enzymes capable of rapid deconstruction of matter. The secretions can be purified and added directly to biomass slurry.

Key Benefits
  • Secretions provide all the enzymes needed for breaking down cellulose to cellobiose in soluble form.
  • Proteins can be purified directly from secretions without tags or recombinant means.
  • ActE is able to grow in a wide range of pH.
  • Bacteria can be genetically modified to achieve proteolysis-proof secretions.
  • Biofuels
  • Converting cellulosic biomass to cellobiose and xylose
  • Converting paper waste to readily fermentable saccharides
  • Animal feeds with easier digestibility
  • Processing shellfish chitin into soluble constituents
  • Converting mannan-enriched material to mannose and mannobiose
  • Commercial food processing
Stage of Development

The development of this technology was supported by the WARF Accelerator Program. The Accelerator Program selects WARF’s most commercially promising technologies and provides expert assistance and funding to enable achievement of commercially significant milestones. WARF believes that these technologies are especially attractive opportunities for licensing.

Technology Contact

For current licensing status, please contact Jennifer Gottwald at jennifer@warf.org or (608) 262-5941.

Sustainable Biomass Conversion