Utilization of lignocellulosic biofuel conversion residue by diverse microorganisms


C. S. Wadler et al. "Utilization of lignocellulosic biofuel conversion residue by diverse microorganisms" Biotechnology for Biofuels and Bioproducts 15 (2022) [DOI:10.1186/s13068-022-02168-0]


Lignocellulosic conversion residue (LCR) is the material remaining after deconstructed lignocellulosic biomass is subjected to microbial fermentation and treated to remove the biofuel. Technoeconomic analyses of biofuel refineries have shown that further microbial processing of this LCR into other bioproducts may help offset the costs of biofuel generation. Identifying organisms able to metabolize LCR is an important first step for harnessing the full chemical and economic potential of this material. In this study, we investigated the aerobic LCR utilization capabilities of 71 Streptomyces and 163 yeast species that could be engineered to produce valuable bioproducts. The LCR utilization by these individual microbes was compared to that of an aerobic mixed microbial consortium derived from a wastewater treatment plant as representative of a consortium with the highest potential for degrading the LCR components and a source of genetic material for future engineering efforts.

Data Access

The raw 16S rRNA gene amplicon sequencing reads are available under NCBI BioProject ID PRJNA751072.

Biomass analytics
Phylogenetic relationships