Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling

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Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling

Publication Type:

Journal Article

Source:

Biotechnology for Biofuels, Volume 10, Number 1, p.31 (2017)

ISBN:

1754-6834

Keywords:

Deconstruction

Abstract:

Glycoside hydrolases (GHs) are enzymes that hydrolyze polysaccharides into simple sugars. To better understand the specificity of enzyme hydrolysis within the complex matrix of polysaccharides found in the plant cell wall, we studied the reactions of individual enzymes using glycome profiling, where a comprehensive collection of cell wall glycan-directed monoclonal antibodies are used to detect polysaccharide epitopes remaining in the walls after enzyme treatment and quantitative nanostructure initiator mass spectrometry (oxime-NIMS) to determine soluble sugar products of their reactions.