Bioprospecting for Cellulose-Degrading Microbes: Individual Isolate Method

Bioprospecting for Cellulose-Degrading Microbes: Individual Isolate Method

isolate method

Students investigate locations they believe harbor cellulose-digesting microbes, collect samples, isolate them on selective media, and screen them for cellulase activity. These novel microbes may be useful for the production of cellulosic ethanol. In the process they learn about plating techniques, serial dilutions, symbiotic relationships and enzyme specificity. Two methods are provided, one focusing on isolation of pure microbial strains, the other focusing on finding symbiotic communities of microbes.

Audience High School through Undergraduate (9-16)
Related Subject Areas Agriculture, Engineering
Prior Knowledge Symbiotic relationships, types of carbohydrates, basic cell wall structure, enzyme structure and function
Time Required Five to seven 50-minute class periods over 12-18 days (can be adapted to short times)
Required Supplies Community method: Erlenmeyer flasks, aluminum foil, cellulosic biomass (i.e. wood chips, corn stover, saw dust, etc), Petri dished (optional), Carboxymethyl Cellulose (optional), Congo Red (optional);  Isolation method:  Centrifuge tubes, Petri Dishes, Carboxymethyl Cellulose (CMC), Microcrystalline Cellulose (optional), Agar, Small pestles, Bacteria spreaders, Inoculating loops, PBS Buffer or TRIS buffer, Congo Red, P200 pipette tips, 10uL dispensing micropipettes, Parafilm, Ethanol, Bleach, NaCl. **See activity package for amounts and supplier details
Tags/Keywords high school, undergraduate, cellulose, microbe, enzyme, bioprospecting, ethanol, interactive, community
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Bioprospecting Package (.zip, 9.8 MB)
Includes: Includes: Teacher Instructions and Answer Key, Student Activities and Worksheet, Supplementary Materials