Bioprospecting for Cellulose-Degrading Microbes: Filter Paper Assay Method
Middle School
              High School
              Undergraduate
          Investigation
          
Students collect samples that they predict will contain communities of cellulose-degrading microbes and test for the ability of microorganisms in their samples to break down pure cellulose (filter paper). In the process, groups collect evidence to test predictions about which environmental microbial samples will be the most effective for degrading cellulose. By comparing results across groups, students can begin to uncover patterns and develop explanations about the types of environments that support cellulose-degrading microbes. This lab method is nearly identical to that used by GLBRC researchers and student results could help scientists discover new enzymes for efficient biofuel production.
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Subjects
          Agriculture
          Biology
          Engineering
          Environmental Science
              Topics
          Biodiversity & Evolution
          Ecosystems
          Fermentation & Enzymes
              Concepts/Skills
              Decomposition, respiration, biomolecules, enzymes, microbial ecology, data analysis, scientific argumentation
          Prior Knowledge
              Type of carbohydrates, basic cell wall structure, enzyme function, role of microorganisms in decomposition, matter and energy transformations
          Time Required
              Five to seven 50-minute class periods over 12-18 days (can be adapted to shorter times)
          Required Supplies
              Test tubes, test tube shaker, filter paper, Miracle Gro fertilizer (see teacher instructions for details and supplier info.)